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human polyclonal anti βactin  (Proteintech)


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    Proteintech human polyclonal anti βactin
    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).
    Human Polyclonal Anti βactin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human polyclonal anti βactin/product/Proteintech
    Average 96 stars, based on 2889 article reviews
    human polyclonal anti βactin - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "High androgen level during controlled ovarian stimulation cycle impairs endometrial receptivity in PCOS patients."

    Article Title: High androgen level during controlled ovarian stimulation cycle impairs endometrial receptivity in PCOS patients.

    Journal: Scientific reports

    doi: 10.1038/s41598-024-74295-7

    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).
    Figure Legend Snippet: Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).

    Techniques Used: Expressing, Binding Assay, Reverse Transcription, Polymerase Chain Reaction, Control, Western Blot



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    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).
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    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).
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    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).
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    Image Search Results


    Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).

    Journal: Scientific reports

    Article Title: High androgen level during controlled ovarian stimulation cycle impairs endometrial receptivity in PCOS patients.

    doi: 10.1038/s41598-024-74295-7

    Figure Lengend Snippet: Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).

    Article Snippet: In brief, the separated samples were transferred to polyvinylidene difluoride membranes and incubated overnight at 4 °C with rabbit polyclonal anti-α-IGFBP-1 (1:1000;13981-1-AP, Proteintech), anti-LIF (1:1000; 26757-1-AP, Proteintech), and human polyclonal anti-βactin (1:0000; 66031-1-Ig Proteintech).

    Techniques: Expressing, Binding Assay, Reverse Transcription, Polymerase Chain Reaction, Control, Western Blot